7/5
I began cloning FSIPPW again.
I added 5 x 106 293 cells onto plates, 5 million on each of four plates with FT media (no antibiotics). I then added 500,000 cells onto an additional plate to grow.
7/6
Today was a really busy day, but you have heard it all before. I annealed the oligos again, CIP treated the FSIPPW, split my clone A cells, ligated the FSIPPW and oligos, then transformed my bacteria.
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